phospho jak2 tyr1007 Search Results


jak2 [p tyr1007, p tyr1008] antibody (sy24-03)  (Bio-Techne corporation)
94
Bio-Techne corporation jak2 [p tyr1007, p tyr1008] antibody (sy24-03)
Jak2 [P Tyr1007, P Tyr1008] Antibody (Sy24 03), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/jak2 [p tyr1007, p tyr1008] antibody (sy24-03)/product/Bio-Techne corporation
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phospho jak2 tyr1007 1008 c80c3  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho jak2 tyr1007 1008 c80c3
PI3K and <t>JAK2</t> signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.
Phospho Jak2 Tyr1007 1008 C80c3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phospho jak2 tyr1007 1008 c80c3/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
phospho jak2 tyr1007 1008 c80c3 - by Bioz Stars, 2026-02
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p jak  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc p jak
PI3K and <t>JAK2</t> signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.
P Jak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p jak/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
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p jak2  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc p jak2
PI3K and <t>JAK2</t> signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.
P Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p jak2/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
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rabbit anti jak2  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc rabbit anti jak2
PI3K and <t>JAK2</t> signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.
Rabbit Anti Jak2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti jak2/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
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jak2 antibodies  (Biorbyt)
92
Biorbyt jak2 antibodies
Fig. 2. Baricitinib and tofacitinib similarly inhibit eosinophil differentiation and JAK1/2 activation. Bone marrow cells were collected from the femurs and tibiae of three female BALB/c mice and cultured at 106/mL in media supplemented with 100 ng/mL SCF and 100 ng/mL FLT3L from day 0 to day 4. On day 4, media were replaced with media containing 10 ng/mL IL-5 supplemented with or without baricitinib (BARI, 200 nM) or tofacitinib (TOFA, 200 nM). (A) Total cells, (B) Siglec F positive cells and (C) CCR3 positive cells were enumerated by flow cytometry on the indicated days. On day 12 (D) JAK1 and (E) <t>JAK2</t> phosphorylation was measured by flow cytometric intracellular staining. Data are shown as mean ± SEM from 3 independent experiments, ** P < 0.01, *** P < 0.005; (A-C) two-way ANOVA and (D and E) one- way ANOVA.
Jak2 Antibodies, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
jak2 antibodies - by Bioz Stars, 2026-02
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phosphorylated tyk2  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc phosphorylated tyk2
Fig. 2. Baricitinib and tofacitinib similarly inhibit eosinophil differentiation and JAK1/2 activation. Bone marrow cells were collected from the femurs and tibiae of three female BALB/c mice and cultured at 106/mL in media supplemented with 100 ng/mL SCF and 100 ng/mL FLT3L from day 0 to day 4. On day 4, media were replaced with media containing 10 ng/mL IL-5 supplemented with or without baricitinib (BARI, 200 nM) or tofacitinib (TOFA, 200 nM). (A) Total cells, (B) Siglec F positive cells and (C) CCR3 positive cells were enumerated by flow cytometry on the indicated days. On day 12 (D) JAK1 and (E) <t>JAK2</t> phosphorylation was measured by flow cytometric intracellular staining. Data are shown as mean ± SEM from 3 independent experiments, ** P < 0.01, *** P < 0.005; (A-C) two-way ANOVA and (D and E) one- way ANOVA.
Phosphorylated Tyk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phosphorylated tyk2/product/Cell Signaling Technology Inc
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Image Search Results


PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.

Journal: International Journal of Molecular Medicine

Article Title: Hepatocyte growth factor inhibits hypoxia/reoxygenation-induced activation of xanthine oxidase in endothelial cells through the JAK2 signaling pathway

doi: 10.3892/ijmm.2016.2708

Figure Lengend Snippet: PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (A) Representative western blot of XO and tubulin expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). LY294002 and AG490 significantly decreased the increase in the XO protein level induced by H/R. (B) Summary data (n=3 biological replicates) of western blot analysis of XO and tubulin expression in CMECs following H/R. LY294002, SB203580 and AG490 treatment group compared with H/R group. * p<0.05 vs. H/R group. PI3K and JAK2 signaling pathways are involved in the production and activation of xanthine oxidase (XO). (C) Representative western blots of phosphorylated (p-)PI3K, total (t-)PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). H/R activates the PI3K and JAK2 signaling pathways but not the p38 MAPK signaling pathway. (D) Summary data (n=3 biological replicates) of western blot analysis of p-PI3K, t-PI3K, p-JAK2, t-JAK2, p-p38 MAPK and t-p38 MAPK expression in CMECs. ** p<0.01 vs. control group. (E) Representative data for flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002, SB203580 and AG490 treatment groups compared with H/R group. (F) Summary data (n=3 biological replicates) of flow cytometric analysis of DCFH-DA-stained CMECs following H/R. LY294002 (1 µ M), SB203580 (30 µ M) and AG490 (30 µ M) treatment groups compared with H/R group. * p<0.05 vs. H/R group; ** p<0.01 vs. H/R group.

Article Snippet: Forty micrograms of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and then probed with antibodies for XO (#ab109235; Abcam), phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199) (#4228), PI3 kinase p85 (19H8) (#4257), phospho-p38 MAP kinase (Thr180/Thr182) (#4631), p38 MAPK (D13E1) (#8690), JAK2 (D2E12) (#3230) and phospho-JAK2 (Tyr1007/1008) (C80C3) (#3776) (all from Cell Signaling Technology, Beverly, MA, USA).

Techniques: Protein-Protein interactions, Activation Assay, Western Blot, Expressing, Control, Staining

PI3K siRNA and JAK2 siRNA downregulate the production and activation of xanthine oxidase (XO). (A) Representative western blots of p38 MAPK, PI3K, JAK2 and tubulin expression in cardiac microvascular endothelial cells (CMECs). (B) Representative western blot of XO and tubulin expression in CMECs following hypoxia/reoxygenation (H/R). PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- and negative control siRNA-transfected groups compared with H/R group. (C) Summary data (n=3 biological replicates) for western blot analysis of XO and tubulin expression in CMECs following H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. ** p<0.01 vs. H/R group. (D) Representative flow cytometric analysis of DCFH-DA-stained CMECs after H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. (E) Summary data (n=3 biological replicates) for flow cytometric analysis of DCFH-DA-stained CMECs after H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. ** p<0.01 vs. H/R group.

Journal: International Journal of Molecular Medicine

Article Title: Hepatocyte growth factor inhibits hypoxia/reoxygenation-induced activation of xanthine oxidase in endothelial cells through the JAK2 signaling pathway

doi: 10.3892/ijmm.2016.2708

Figure Lengend Snippet: PI3K siRNA and JAK2 siRNA downregulate the production and activation of xanthine oxidase (XO). (A) Representative western blots of p38 MAPK, PI3K, JAK2 and tubulin expression in cardiac microvascular endothelial cells (CMECs). (B) Representative western blot of XO and tubulin expression in CMECs following hypoxia/reoxygenation (H/R). PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- and negative control siRNA-transfected groups compared with H/R group. (C) Summary data (n=3 biological replicates) for western blot analysis of XO and tubulin expression in CMECs following H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. ** p<0.01 vs. H/R group. (D) Representative flow cytometric analysis of DCFH-DA-stained CMECs after H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. (E) Summary data (n=3 biological replicates) for flow cytometric analysis of DCFH-DA-stained CMECs after H/R. PI3K siRNA-, p38 MAPK siRNA-, JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. ** p<0.01 vs. H/R group.

Article Snippet: Forty micrograms of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and then probed with antibodies for XO (#ab109235; Abcam), phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199) (#4228), PI3 kinase p85 (19H8) (#4257), phospho-p38 MAP kinase (Thr180/Thr182) (#4631), p38 MAPK (D13E1) (#8690), JAK2 (D2E12) (#3230) and phospho-JAK2 (Tyr1007/1008) (C80C3) (#3776) (all from Cell Signaling Technology, Beverly, MA, USA).

Techniques: Activation Assay, Western Blot, Expressing, Negative Control, Transfection, Staining

Hepatocyte growth factor (HGF) inhibits activation of the JAK2 signaling pathway but has no effect on the PI3K signaling pathway. (A) Representative western blot of phosphorylated (p-)JAK2 and total (t-)JAK2 expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). HGF significantly decreased the H/R-induced activation of the JAK2 pathway. (B) Summary data (n=3 biological replicates) for western blot analysis of p-JAK2 and t-JAK2 in CMECs after H/R. HGF (10 and 20 ng/ml) treatment groups compared with H/R group. ** p<0.01 vs. H/R group. (C) Representative western blot of p-PI3K and t-PI3K expression in CMECs after H/R. HGF had no effect on the PI3K signaling pathway. (D) Summary data (n=3 biological replicates) for western blot analysis of p-PI3K and t-PI3K expression in CMECs following H/R.

Journal: International Journal of Molecular Medicine

Article Title: Hepatocyte growth factor inhibits hypoxia/reoxygenation-induced activation of xanthine oxidase in endothelial cells through the JAK2 signaling pathway

doi: 10.3892/ijmm.2016.2708

Figure Lengend Snippet: Hepatocyte growth factor (HGF) inhibits activation of the JAK2 signaling pathway but has no effect on the PI3K signaling pathway. (A) Representative western blot of phosphorylated (p-)JAK2 and total (t-)JAK2 expression in cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). HGF significantly decreased the H/R-induced activation of the JAK2 pathway. (B) Summary data (n=3 biological replicates) for western blot analysis of p-JAK2 and t-JAK2 in CMECs after H/R. HGF (10 and 20 ng/ml) treatment groups compared with H/R group. ** p<0.01 vs. H/R group. (C) Representative western blot of p-PI3K and t-PI3K expression in CMECs after H/R. HGF had no effect on the PI3K signaling pathway. (D) Summary data (n=3 biological replicates) for western blot analysis of p-PI3K and t-PI3K expression in CMECs following H/R.

Article Snippet: Forty micrograms of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and then probed with antibodies for XO (#ab109235; Abcam), phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199) (#4228), PI3 kinase p85 (19H8) (#4257), phospho-p38 MAP kinase (Thr180/Thr182) (#4631), p38 MAPK (D13E1) (#8690), JAK2 (D2E12) (#3230) and phospho-JAK2 (Tyr1007/1008) (C80C3) (#3776) (all from Cell Signaling Technology, Beverly, MA, USA).

Techniques: Activation Assay, Western Blot, Expressing

JAK2 siRNA downregulates cytosolic calcium concentrations. (A) Representative data for flow cytometric analysis of fluo-3-stained cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. (B) Summary data (n=3 biological replicates) for flow cytometric analysis of fluo-3-stained CMECs after H/R. JAK2 siRNA- or negative control siRNA-transfected group compared with H/R group. ** p<0.01 vs. H/R group.

Journal: International Journal of Molecular Medicine

Article Title: Hepatocyte growth factor inhibits hypoxia/reoxygenation-induced activation of xanthine oxidase in endothelial cells through the JAK2 signaling pathway

doi: 10.3892/ijmm.2016.2708

Figure Lengend Snippet: JAK2 siRNA downregulates cytosolic calcium concentrations. (A) Representative data for flow cytometric analysis of fluo-3-stained cardiac microvascular endothelial cells (CMECs) following hypoxia/reoxygenation (H/R). JAK2 siRNA- or negative control siRNA-transfected groups compared with H/R group. (B) Summary data (n=3 biological replicates) for flow cytometric analysis of fluo-3-stained CMECs after H/R. JAK2 siRNA- or negative control siRNA-transfected group compared with H/R group. ** p<0.01 vs. H/R group.

Article Snippet: Forty micrograms of protein was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes, and then probed with antibodies for XO (#ab109235; Abcam), phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199) (#4228), PI3 kinase p85 (19H8) (#4257), phospho-p38 MAP kinase (Thr180/Thr182) (#4631), p38 MAPK (D13E1) (#8690), JAK2 (D2E12) (#3230) and phospho-JAK2 (Tyr1007/1008) (C80C3) (#3776) (all from Cell Signaling Technology, Beverly, MA, USA).

Techniques: Staining, Negative Control, Transfection

Fig. 2. Baricitinib and tofacitinib similarly inhibit eosinophil differentiation and JAK1/2 activation. Bone marrow cells were collected from the femurs and tibiae of three female BALB/c mice and cultured at 106/mL in media supplemented with 100 ng/mL SCF and 100 ng/mL FLT3L from day 0 to day 4. On day 4, media were replaced with media containing 10 ng/mL IL-5 supplemented with or without baricitinib (BARI, 200 nM) or tofacitinib (TOFA, 200 nM). (A) Total cells, (B) Siglec F positive cells and (C) CCR3 positive cells were enumerated by flow cytometry on the indicated days. On day 12 (D) JAK1 and (E) JAK2 phosphorylation was measured by flow cytometric intracellular staining. Data are shown as mean ± SEM from 3 independent experiments, ** P < 0.01, *** P < 0.005; (A-C) two-way ANOVA and (D and E) one- way ANOVA.

Journal: Biochemical pharmacology

Article Title: The JAK1/2 inhibitor baricitinib suppresses eosinophil effector function and restricts allergen-induced airway eosinophilia.

doi: 10.1016/j.bcp.2021.114690

Figure Lengend Snippet: Fig. 2. Baricitinib and tofacitinib similarly inhibit eosinophil differentiation and JAK1/2 activation. Bone marrow cells were collected from the femurs and tibiae of three female BALB/c mice and cultured at 106/mL in media supplemented with 100 ng/mL SCF and 100 ng/mL FLT3L from day 0 to day 4. On day 4, media were replaced with media containing 10 ng/mL IL-5 supplemented with or without baricitinib (BARI, 200 nM) or tofacitinib (TOFA, 200 nM). (A) Total cells, (B) Siglec F positive cells and (C) CCR3 positive cells were enumerated by flow cytometry on the indicated days. On day 12 (D) JAK1 and (E) JAK2 phosphorylation was measured by flow cytometric intracellular staining. Data are shown as mean ± SEM from 3 independent experiments, ** P < 0.01, *** P < 0.005; (A-C) two-way ANOVA and (D and E) one- way ANOVA.

Article Snippet: On day 12, cells were processed with Fix & Perm (Nordic MUbio) and additionally stained with FITC anti-mouse phospho-JAK1 and JAK2 antibodies (both Biorbyt), evaluated by flow cytometry (BD FACSCanto II) and and analyzed by FlowJo 10.7.1 software.

Techniques: Activation Assay, Cell Culture, Flow Cytometry, Phospho-proteomics, Staining